The coomassie brilliant blue protein assay commonly known as the bradford assay 1 is widely used because of its rapid and convenient protocol as well as its relative sensitivity unfortunately there is a large degree of curvature over a broad range of protein concentrations fig. The micro assay has a linear range of 15 100 µg ml using a 1 1 sample to reagent ratio. The standard assay has a higher range of 100 1000 µg ml which may be obtained using a 1 30.
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